Journal
/
Immunologie und Infektion
/
Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
JoVE Journal
Immunologie und Infektion
Author Produced
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

9,564 Views

•

10:07 min

•

July 15, 2018

DOI:

10.3791/57740-v

DOI:
10.3791/57740-v

•

10:07 min
July 15, 2018

•

5 Views
, , , ,
1Department of Genetics, Physical Anthropology and Animal Physiology,University of the Basque Country (UPV-EHU), Biocruces Health Research Institute, 2Endocrinology and Diabetes Research Group, Biocruces Health Research Institute,UPV-EHU, CIBERDEM

Summary

Automatically generated

Here, we present a protocol to perform a simple cellular fractionation for the subcellular separation of cytoplasmic and nuclear proteins in human fresh and frozen intestinal biopsies.

Explore More Videos

Subcellular FractionationGastrointestinal SpecimensProtein LocalizationIntestinal BiopsyNuclear And Cytoplasmic FractionsHomogenizationDetergent LysisCentrifugation

Read Article

Cite this Article

Romero-Garmendia, I., Jauregi-Miguel, A., Santin, I., Bilbao, J. R., Castellanos-Rubio, A. Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens. J. Vis. Exp. (137), e57740, doi:10.3791/57740 (2018).

Download .ris file

Copy

Share Video

.

Copy