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Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
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JoVE 杂志 免疫与感染
Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

9,564 Views

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10:07 min

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July 15, 2018

DOI:

10.3791/57740-v

DOI:
10.3791/57740-v

•

10:07 min
July 15, 2018

•

5 Views
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1Department of Genetics, Physical Anthropology and Animal Physiology,University of the Basque Country (UPV-EHU), Biocruces Health Research Institute, 2Endocrinology and Diabetes Research Group, Biocruces Health Research Institute,UPV-EHU, CIBERDEM

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Here, we present a protocol to perform a simple cellular fractionation for the subcellular separation of cytoplasmic and nuclear proteins in human fresh and frozen intestinal biopsies.

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Subcellular FractionationGastrointestinal SpecimensProtein LocalizationIntestinal BiopsyNuclear And Cytoplasmic FractionsHomogenizationDetergent LysisCentrifugation

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Romero-Garmendia, I., Jauregi-Miguel, A., Santin, I., Bilbao, J. R., Castellanos-Rubio, A. Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens. J. Vis. Exp. (137), e57740, doi:10.3791/57740 (2018).

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