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成年日本血吸虫细胞外囊泡的分离与鉴定
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
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成年日本血吸虫细胞外囊泡的分离与鉴定

7,745 Views

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07:37 min

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May 22, 2018

DOI:

10.3791/57514-v

DOI:
10.3791/57514-v

•

07:37 min
May 22, 2018

•

4 Views
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1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology,Ministry of Agriculture, 2Tianjin Agricultural University

Summary

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在这里, 我们提出一个协议, 以描述细胞外囊泡 (电动车) 隔离在体外培养培养基从成年日本血吸虫。

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Extracellular VesiclesSchistosoma JaponicumIn Vitro CultureIsolationPurificationCentrifugationDialysisPEG8000Western Blot

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Liu, J., Zhu, L., Wang, L., Chen, Y., Giri, B. R., Li, J., Cheng, G. Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum. J. Vis. Exp. (135), e57514, doi:10.3791/57514 (2018).

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