Journal
/
/
Rendering tridimensionale e l'analisi di Immunolabeled, chiarito reti vascolari Villous placentare umani
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
DOI:

09:33 min

March 29, 2018

, , , , , , , ,

Kapitel

  • 00:04Titel
  • 01:17Villous Tree Dissection and Immunofluorescent Staining
  • 03:16Clarification of the Tissue
  • 04:07Mounting for Microscopy and Confocal Microscopy
  • 06:04Reversing the Clearing
  • 06:33Results: Use of Confocal Microscopy, Immunostaining, and Reverse Clearing to Analyze the Vascularization of Placental Network
  • 08:21Conclusion

Summary

Automatische Übersetzung

Questo studio presenta un protocollo per il tessuto reversibile di compensazione, immunostaining, 3D-rendering e analisi di reti vascolari nei campioni di villi di placenta umana nell'ordine di 1-2 mm3.

Verwandte Videos

Read Article