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Developmental Biology

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JoVE Journal Developmental Biology

单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析

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单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析

Article DOI: 10.3791/56973-v • 09:32 min • May 8th, 2018

May 8th, 2018 •

Brandon K. Hadland^1,2, Barbara Varnum-Finney¹, Cynthia Nourigat-Mckay¹, David Flowers¹, Irwin D. Bernstein^1,2

¹Clinical Research Division, Fred Hutchinson Cancer Research Center, ²Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Washington School of Medicine

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本文介绍了小鼠胚胎发育过程中造血干细胞前体的克隆分析方法。我们结合内皮细胞共培养和移植对胚胎主动脉-性腺-中区单细胞的指标进行分类, 以表征单造血前体的表型性质和植入电位。

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发育生物学问题 135 造血干细胞 (hsc) 前 HSC 主动脉-性腺-中区 (年会) 胚胎造血指数排序单细胞克隆分析造血干细胞移植

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