Journal
/
Entwicklungsbiologie
/
Klonede analyse af embryonale hæmatopoietisk stamcelle prækursorer ved hjælp af enkelt celle indeks sortering kombineret med endotel celle Niche Co kultur
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Klonede analyse af embryonale hæmatopoietisk stamcelle prækursorer ved hjælp af enkelt celle indeks sortering kombineret med endotel celle Niche Co kultur

8,414 Views

•

09:32 min

•

May 08, 2018

DOI:

10.3791/56973-v

DOI:
10.3791/56973-v

•

09:32 min
May 08, 2018

•

3 Views
, , , ,
1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology,University of Washington School of Medicine

Tags

Clonal AnalysisEmbryonic Hematopoietic Stem Cell PrecursorsSingle Cell Index SortingEndothelial Cell Niche Co-cultureDevelopmental HematopoiesisLineage ContributionsHemogenic PrecursorsPhenotypic PropertiesHSC PrecursorsCollagenaseAntibody CocktailFlow CytometrySingle Cell SortingIndex Sorting

Read Article

Cite this Article

Hadland, B. K., Varnum-Finney, B., Nourigat-Mckay, C., Flowers, D., Bernstein, I. D. Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture. J. Vis. Exp. (135), e56973, doi:10.3791/56973 (2018).

Download .ris file

Copy

Share Video

.

Copy