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Met behulp van 3 Single-molecuul FRET te bestuderen de correlatie van eiwitinteractie
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JoVE Journal Biochemie
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
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Met behulp van 3 Single-molecuul FRET te bestuderen de correlatie van eiwitinteractie

9,796 Views

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11:22 min

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January 30, 2018

DOI:

10.3791/56896-v

DOI:
10.3791/56896-v

•

11:22 min
January 30, 2018

•

1 Views
, , ,
1Institute of Physical Chemistry,University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Tags

Three-color Single-molecule FRETProtein InteractionsCooperativityQuantitative BiologyProtein ComplexesDynamic InteractionsFlow Chamber AssemblyPEG-biotin Functionalized SlideMulti-color Prism TIRF MicroscopeEMCCD Camera Configuration

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Götz, M., Wortmann, P., Schmid, S., Hugel, T. Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions. J. Vis. Exp. (131), e56896, doi:10.3791/56896 (2018).

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