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Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

DOI:
10.3791/56768-v

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10:25 min

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January 23, 2018

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, , , ,
1Institute of Reconstructive Neurobiology,University of Bonn, 2Central Institute of Mental Health,University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)

Kapitel

  • 00:05Titel
  • 00:55iPSC Aggregate Generation
  • 02:30Anterior Neuroectoderm Induction
  • 03:51Neuroectodermal Aggregates embedding within a Matrix Scaffold
  • 05:49Forebrain-type Organoid Generation, Fixation, and Validation
  • 07:58Results: Representative Forebrain-type Organoid Validation
  • 09:36Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Cerebral organoids represent a new model system to investigate early human brain development in vitro. This article provides the detailed methodology to efficiently generate homogeneous dorsal forebrain-type organoids from human induced pluripotent stem cells including critical characterization and validation steps.

Tags

Human Induced Pluripotent Stem CellsForebrain-type Cerebral OrganoidsStandardized And Reproducible Organoid GenerationModeling NeurodevelopmentCortical GenesisAggregationAnterior Neuroectoderm Induction

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