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Standart ve tekrarlanabilir ön türü beyin Organoids insan nesil Pluripotent kök hücreler indüklenen
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
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Standart ve tekrarlanabilir ön türü beyin Organoids insan nesil Pluripotent kök hücreler indüklenen

20,682 Views

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10:25 min

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January 23, 2018

DOI:

10.3791/56768-v

DOI:
10.3791/56768-v

•

10:25 min
January 23, 2018

•

21 Views
, , , ,
1Institute of Reconstructive Neurobiology,University of Bonn, 2Central Institute of Mental Health,University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)

Summary

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Serebral organoids erken insan beyni geliştirme vitroaraştırmak için yeni bir modeli sistemi temsil eder. Bu makale verimli bir şekilde homojen dorsal ön-tipi organoids İnsan İndüklenmiş pluripotent kök hücre de dahil olmak üzere kritik karakterizasyonu ve doğrulama adımları oluşturmak için ayrıntılı yöntembilim sağlar.

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Cite this Article

Krefft, O., Jabali, A., Iefremova, V., Koch, P., Ladewig, J. Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (131), e56768, doi:10.3791/56768 (2018).

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