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Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

20,742 Views

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10:25 min

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January 23, 2018

DOI:

10.3791/56768-v

DOI:
10.3791/56768-v

•

10:25 min
January 23, 2018

•

21 Views
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1Institute of Reconstructive Neurobiology,University of Bonn, 2Central Institute of Mental Health,University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)

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Cerebral organoids represent a new model system to investigate early human brain development in vitro. This article provides the detailed methodology to efficiently generate homogeneous dorsal forebrain-type organoids from human induced pluripotent stem cells including critical characterization and validation steps.

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Krefft, O., Jabali, A., Iefremova, V., Koch, P., Ladewig, J. Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (131), e56768, doi:10.3791/56768 (2018).

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