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Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
Kapitel
- 00:04Titel
- 00:59Harvesting Fetal or Neonatal Ovaries
- 03:55Metaphase I (MI) or Metaphase II (MII) Oocyte Collection
- 05:17Oocyte Denuding and Zona Pellucida Removal
- 07:05MI and MII Oocyte Chromatin Spreads
- 08:08Results: Crossover Formation in C57B/6J Oocytes
- 09:52Conclusion
Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.
