Journal
/
Genetik
/
从前期到中期二期小鼠卵母细胞进展的染色质传播制剂分析
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
Please note that all translations are automatically generated. Click here for the English version.

从前期到中期二期小鼠卵母细胞进展的染色质传播制剂分析

14,895 Views

•

10:39 min

•

February 26, 2018

DOI:

10.3791/56736-v

DOI:
10.3791/56736-v

•

10:39 min
February 26, 2018

•

13 Views
, ,
1Department of Biochemistry and Molecular Biology,Johns Hopkins University Bloomberg School of Public Health

Summary

Automatically generated

哺乳动物中的卵子发生过程已知易出错, 特别是由于染色体 missegregation。本手稿描述了小鼠前期、中期 I 和 II. 期卵母细胞的染色质传播制备方法。这些基本技术允许研究整个哺乳动物卵子发生过程的染色质束缚蛋白和染色体形态学。

Explore More Videos

Chromatin SpreadMouse OocyteProphaseMetaphase IIOogenesisChromosomeImmunolabelingPloidyAneuploidyInfertilityOvaryHypo-extraction BufferSucrose

Read Article

Cite this Article

Hwang, G. H., Hopkins, J. L., Jordan, P. W. Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II. J. Vis. Exp. (132), e56736, doi:10.3791/56736 (2018).

Download .ris file

Copy

Share Video

.

Copy