Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.
Hwang, G. H., Hopkins, J. L., Jordan, P. W. Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II. J. Vis. Exp. (132), e56736, doi:10.3791/56736 (2018).