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An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
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JoVE Journal Genetik
An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

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09:32 min

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November 08, 2017

DOI:

10.3791/56470-v

DOI:
10.3791/56470-v

•

09:32 min
November 08, 2017

•

1 Views
, , , , ,
1Laboratory of Plant Genetics and Development,Noble Research Institute, 2Genetics Laboratory,University of Oklahoma Health Science Center, 3Medicine and Health School,Li Shui University

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This protocol provides experimental steps and information about reagents, equipment, and analysis tools for researchers who are interested in carrying out whole genome array-based comparative genomic hybridization (CGH) analysis of copy number variations in plants.

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Array-based Comparative Genomic HybridizationCopy Number VariationsFast Neutron-induced MutantsMedicago TruncatulaLegume BiologyNodule DevelopmentDNA ExtractionDNA LabelingDNA Purification

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Chen, Y., Wang, X., Lu, S., Wang, H., Li, S., Chen, R. An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants. J. Vis. Exp. (129), e56470, doi:10.3791/56470 (2017).

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