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An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
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Genetics
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JoVE Journal Genetics
An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

DOI:
10.3791/56470-v

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09:32 min

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November 08, 2017

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, , , , ,
1Laboratory of Plant Genetics and Development,Noble Research Institute, 2Genetics Laboratory,University of Oklahoma Health Science Center, 3Medicine and Health School,Li Shui University

Chapters

  • 00:05Title
  • 01:00DNA Isolation, Labeling, and Purification
  • 04:04Hybridization, Washing, and Scanning of the Genome Arrays
  • 06:45Results: Array-based Comparative Genome Hybridization to Detect Copy Number Variations in Medicago truncatula Mutants
  • 08:09Conclusion

Summary

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This protocol provides experimental steps and information about reagents, equipment, and analysis tools for researchers who are interested in carrying out whole genome array-based comparative genomic hybridization (CGH) analysis of copy number variations in plants.

Tags

Array-based Comparative Genomic HybridizationCopy Number VariationsFast Neutron-induced MutantsMedicago TruncatulaLegume BiologyNodule DevelopmentDNA ExtractionDNA LabelingDNA Purification

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