Journal
/
Entwicklungsbiologie
/
全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化
JoVE Journal
Entwicklungsbiologie
This content is Free Access.
JoVE Journal Entwicklungsbiologie
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
Please note that all translations are automatically generated. Click here for the English version.

全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化

DOI:
10.3791/56261-v

•

09:33 min

•

November 18, 2017

•

, , , ,
1Department of Biological Sciences,University of Notre Dame

Kapitel

  • 00:05Titel
  • 00:50Embryo Fixation and Hybridization
  • 03:05Hot Washes, Blocking, Antibody Incubation, and Maleic Acid Buffer Washes
  • 04:27Probe Detection, Peroxidase Removal, and Immunofluorescence
  • 07:19Zebrafish Pronephros Mounting and Imaging
  • 08:23Results: Representative Zebrafish Pronephros and Multiciliated Cell (MCC) Imaging
  • 09:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

纤毛发育对正常器官的形成至关重要。该协议描述了一种优化的方法来标记和可视化纤毛细胞的斑马鱼。

Tags

Multiciliated CellsZebrafishWhole Mount Fluorescent In Situ HybridizationImmunofluorescenceEntwicklungsbiologiePronephrosCiliaRNA TranscriptsProtein LabelingAntibodiesFixationRehydrationProteinase KHybridization SolutionRNA Probe

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

了解早期器官使用简体<em>原位</em>杂交协议<em>爪</em

多目标显色全安装<em>原位</em>在比较基因表达域杂交<em>果蝇</em>胚胎

方法来检查淋巴腺和血细胞中<em>果蝇</em>幼虫

器官培养和鼠标沃尔弗导管的全山免疫染色

水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案

小鼠胚胎心肌内祖群的定量全贴免疫荧光分析

培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

斑马鱼原位杂交染色的基因分型与定量

斑马鱼胚胎全山原位杂交和管状测定在iPSC-ECs中研究恩多林在血管发育中的作用

Read Article