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Riprogrammazione primario del liquido amniotico e le celle a membrana di pluripotenza in condizioni prive di Xeno
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1Mitchell Cancer Institute,University of South Alabama, 2College of Medicine,University of South Alabama, 3Institute for Regenerative Medicine,University of Zurich, 4Department of Dermatology,University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM),University of Zurich – Irchel Campus
Kapitel
- 00:05Titel
- 00:50Isolation of Primary Mesenchymal Stem Cells from Human Amnion
- 02:31Harvesting Cultured Amniotic Fluid Stem Cells and Amniotic Membrane Stem Cells
- 03:47Transfection with Reprogramming Plasmids
- 05:35Picking Fully Reprogrammed Colonies for Clonial Expansion
- 06:43Clonal Expansion and Maturation of iPSCs
- 07:47Results: Morphological Progression of Cells During Reprogramming
- 08:43Conclusion
Questo protocollo descrive la riprogrammazione di primaria amniotico liquido e membrana di cellule staminali mesenchimali in cellule staminali pluripotenti indotte utilizzando un approccio episomal non-integrazione in condizioni completamente chimicamente definite. Procedure di estrazione, cultura, riprogrammazione e caratterizzazione delle cellule staminali pluripotenti indotte risultanti dai rigorosi metodi sono dettagliate.
Tags
Keyword Extraction:Amniotic FluidMembrane CellsPluripotencyXeno-free ConditionsInduced Pluripotent Stem CellsEpisomal ReprogrammingFetal Stem CellsDisease ModelingBasic ResearchLongitudinal StudiesChemically Defined ConditionsPediatric Cell-based TherapiesPlacentasAmnionRPMI-1640 MediumAFMC MediumAmniotic Fluid Stem CellsMembrane Stem CellsCell Detachment EnzymePBS
