Journal
/
Immunologie und Infektion
/
Zeer Multiplexed, Superresolutie Imaging van T-cellen Met behulp van madSTORM
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
Please note that all translations are automatically generated. Click here for the English version.

Zeer Multiplexed, Superresolutie Imaging van T-cellen Met behulp van madSTORM

7,228 Views

•

08:43 min

•

June 24, 2017

DOI:

10.3791/55997-v

DOI:
10.3791/55997-v

•

08:43 min
June 24, 2017

•

4 Views
, , , , ,
1Laboratory of Cellular & Molecular Biology, National Cancer Institute,National Institutes of Health, 2Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute,National Institutes of Health

Summary

Automatically generated

We tonen een methode aan om meerdere molecuelen te identificeren binnen heterogene nano-structuren bij nauwkeurige molecuul nauwkeurigheid, met behulp van sequentiële binding en elutie van fluorescent gelabelde antilichamen.

Explore More Videos

T CellsMadSTORMSuper-resolution ImagingMultiplexed ImagingSingle Molecule Localization MicroscopyAlexa 647Poly-L-lysineFluorescent NanodiamondsAnti-CD3 AntibodyJurkat T CellsHBS Solution

Read Article

Cite this Article

Yi, J., Manna, A., Barr, V. A., Hong, J., Neuman, K. C., Samelson, L. E. Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM. J. Vis. Exp. (124), e55997, doi:10.3791/55997 (2017).

Download .ris file

Copy

Share Video

.

Copy