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使用madSTORM进行高分辨率，超分辨率的T细胞成像
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
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使用madSTORM进行高分辨率，超分辨率的T细胞成像

7,228 Views

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08:43 min

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June 24, 2017

DOI:

10.3791/55997-v

DOI:
10.3791/55997-v

•

08:43 min
June 24, 2017

•

4 Views
, , , , ,
1Laboratory of Cellular & Molecular Biology, National Cancer Institute,National Institutes of Health, 2Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute,National Institutes of Health

Summary

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我们展示了使用荧光标记抗体的顺序结合和洗脱以单分子精确度在异质纳米结构内成像多个分子的方法。

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Cite this Article

Yi, J., Manna, A., Barr, V. A., Hong, J., Neuman, K. C., Samelson, L. E. Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM. J. Vis. Exp. (124), e55997, doi:10.3791/55997 (2017).

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