Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Matthew Laaper; Takrima Haque; Ruth S. Slack; Arezu Jahani-Asl

doi:10.3791/55871

Journal

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神经科学

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Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

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神经科学

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JoVE 杂志神经科学

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

7,912 Views

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10:36 min

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November 06, 2017

DOI:

10.3791/55871-v

DOI:

10.3791/55871-v

•

10:36 min

November 06, 2017

•

7 Views

Matthew Laaper^1,2, Takrima Haque¹, Ruth S. Slack³, Arezu Jahani-Asl^1,2,4

¹Lady Davis Institute for Medical Research,Jewish General Hospital, ²Integrated Program in Neuroscience,McGill University, ³Department of Cellular and Molecular Medicine,University of Ottawa, ⁴Department of Oncology, Faculty of Medicine,McGill University

Summary

This protocol describes a simple method for isolating and culturing primary mouse cerebral granule neurons (CGNs) from 6-7 day old pups, efficient transduction of CGNs for loss and gain of function studies, and modelling NMDA-induced neuronal excitotoxicity, low-potassium-induced cell death, DNA-damage, and oxidative stress using the same culture model.

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Cerebellar Granule Neurons Neuronal Death Neurodegeneration Primary Cell Culture Excitotoxicity Oxidative Stress DNA Damage Developmental Events Neuronal Activity Morphogenesis Growth Factors Mouse Brain Dissection Cerebellum Isolation Meningeal Removal

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Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

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