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Neuroscience

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JoVE Journal Neuroscience
Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
 

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

Article DOI: 10.3791/55830-v • 11:56 min • June 16th, 2017
June 16th, 2017 •
1
1Department of Molecular Biology, Chonbuk National University

Kapitel

Summary
Automatic Translation

This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.

Tags

Axonal Projection Pattern Embryonic Motor Neurons Drosophila Neural Development Motor Axon Guidance Subcu Formation Visualization Technique Reliable Analysis Axonal Pathfinding Target Condition Defect Egg Collection Plates Yeast Paste Incubate At 25 Degrees Celsius PBT Solution Cotton Swab Micro Centrifuge Tube Decoreonate Embryos Bleach Rinse Heptane Paraformaldehyde
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