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Journal / Developmental Biology / 二维和三维胚体小鼠胚胎干细胞的维甲酸诱导神经分化的分析…
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JoVE Journal Developmental Biology
二维和三维胚体小鼠胚胎干细胞的维甲酸诱导神经分化的分析
 
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二维和三维胚体小鼠胚胎干细胞的维甲酸诱导神经分化的分析

Article DOI: 10.3791/55621-v • 09:04 min • April 22nd, 2017
April 22nd, 2017 •
1, 2, 1, 1,3
1Department of Medicine, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University, 2Department of Molecular Cardiology, Cleveland Clinic Foundation, 3Department of Cancer Biology, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University

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Summary
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我们描述了使用小鼠胚胎干细胞生成二维或三维的胚状体的技术。然后，我们解释如何以诱导胚状体细胞视黄酸，以及如何的神经分化由祖细胞标志物的免疫荧光和免疫印迹来分析其分化状态。

Tags

发育生物学，第122，小鼠胚胎干细胞，神经分化，视黄酸，类胚体，神经祖细胞，免疫荧光，巢蛋白，微管蛋白β3
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