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Kapitel
- 00:05Titel
- 02:00Three Dimensional Culture of Human Neural Stem Cells Using 12-well Plates
- 05:04Evaluation of Changes in miRNA Expression by miRNA PCR Array
- 06:51Computational Analysis for miRNA Target Prediction
- 09:01Results: Genes Targeted by miRNA are Identical from 3D Cultures of hNSCs
- 10:23Conclusion
With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.
