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Detecção da expressão de microRNA na Membrana Peritoneal de Ratos Usando PCR Quantitativa em Tempo Real
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JoVE Journal Genetik
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Detecção da expressão de microRNA na Membrana Peritoneal de Ratos Usando PCR Quantitativa em Tempo Real

7,718 Views

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08:56 min

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June 27, 2017

DOI:

10.3791/55505-v

DOI:
10.3791/55505-v

•

08:56 min
June 27, 2017

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5 Views
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1Division of Nephrology, First Department of Integrated Medicine,Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University

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Keyword Extraction:MiRNAPeritoneal MembraneQuantitative Real-time PCRRNA PurificationRatHomogenizationPhenol-guanidineChloroformEthanolSpin Column

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Hirai, K., Yoshizawa, H., Imai, T., Igarashi, Y., Hirahara, I., Ookawara, S., Ishibashi, K., Morishita, Y. Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR. J. Vis. Exp. (124), e55505, doi:10.3791/55505 (2017).

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