Journal
/
Neurowissenschaften
/
Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils

Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils

DOI:
10.3791/55503-v

•

09:32 min

•

April 13, 2017

•

, , , , ,
1German Center for Neurodegenerative Diseases (DZNE), 2Centre for Prions and Protein Folding Diseases & Department of Biochemistry,University of Alberta

Kapitel

  • 00:05Titel
  • 00:57Intraglossal Injection of Alpha-synuclein Fibrils
  • 02:26Intraperitoneal Injections
  • 03:18Bioluminescence Imaging
  • 06:16Results: Injection wth Alph-synuclein Fibrils via the Intraperitoneal or Intraglossal Route Causes Disease in Biogenic Tg(M83+/-:Gfap-luc+/- Mice
  • 08:28Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Peripheral injection of alpha-synuclein fibrils into the peritoneum or tongue of Tg(M83+/-:Gfap-luc+/-) mice, which express human alpha-synuclein with the familial A53T mutation and firefly luciferase, can induce neuropathology, including neuroinflammation, in their central nervous system.

Tags

Bioluminescence ImagingNeuroinflammationTransgenic MiceAlpha-synuclein FibrilsParkinson’s DiseaseNoninvasive MonitoringAnesthesiaIntranasal InoculationIntraperitoneal InjectionNerve InflammationNeurological Disease

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

Oral Administration of Rotenone using a Gavage and Image Analysis of Alpha-synuclein Inclusions in the Enteric Nervous System

Live Imaging of Dorsal Root Axons after Rhizotomy

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

Using Optical Coherence Tomography and Optokinetic Response As Structural and Functional Visual System Readouts in Mice and Rats

Read Article