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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
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May 25, 2017
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Antibiotic efficacy is most commonly determined by conducting killing kinetic studies and measuring colony forming units (CFUs). By integrating scanning electron microscopy (SEM) with these standard methods, we can distinguish the pharmacological effects of treatment between different antibiotics.
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Endres, B., Bassères, E., Rashid, T., Chang, L., Alam, M. J., Garey, K. W. A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment. J. Vis. Exp. (123), e55383, doi:10.3791/55383 (2017).
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