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Immunology and Infection

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JoVE Journal Immunology and Infection
A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
 

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Article DOI: 10.3791/55383-v • 12:58 min • May 25th, 2017
May 25th, 2017 •
1, 1, 1, 2, 1, 1
1Department of Pharmacy Practice and Translational Research, University of Houston College of Pharmacy, 2Department of Electrical and Computer Engineering, University of Houston

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Antibiotic efficacy is most commonly determined by conducting killing kinetic studies and measuring colony forming units (CFUs). By integrating scanning electron microscopy (SEM) with these standard methods, we can distinguish the pharmacological effects of treatment between different antibiotics.

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Protocol Characterize Morphological Changes Clostridium Difficile Antibiotic Treatment Microbiological Imaging Method Phenotypic Effects Pharmacological Treatment Infective Diseases Morphological Effects Antibiotics C.Difficile High Resolution Imaging In Vitro Cell Culture Techniques Pharmaceutical Killing Action Therapy Clostridium Difficile Infection CDI Identifying Beneficial Insight Pharmacological Action Mixed Culture Model In Vivo Animal Studies Culturing C.difficile Scanning Electron Microscope Antibiotics' Modes Of Actions Research Team Jahangir Alam Professor Microbiologist Lab Activities Tasnuva Rashid
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