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마우스에서 차 간세포 문화에 염증 반응의 유도
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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마우스에서 차 간세포 문화에 염증 반응의 유도

9,762 Views

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08:32 min

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March 10, 2017

DOI:

10.3791/55319-v

DOI:
10.3791/55319-v

•

08:32 min
March 10, 2017

•

3 Views
, , , , ,
1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Summary

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여기서는 성인 마우스로부터 일차 간세포를 분리하는 효소 방법을 보여, 우리는 ELISA 및 실시간 PCR을 이용하여 염증 반응을 정량 설명한다.

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Cite this Article

Czaya, B., Singh, S., Yanucil, C., Schramm, K., Faul, C., Grabner, A. Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice. J. Vis. Exp. (121), e55319, doi:10.3791/55319 (2017).

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