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生後心筋細胞における細胞周期変化の可視化と核の決意
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

生後心筋細胞における細胞周期変化の可視化と核の決意

DOI:

09:41 min

February 24, 2017

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Kapitel

  • 00:05Titel
  • 00:53Cardiomyocyte Isolation
  • 02:52Cardiomyocyte siRNA Transfection and Confocal Video Microscopy Analysis
  • 06:52Results: Representative Cardiomyocyte Analyses
  • 08:59Conclusion

Summary

Automatische Übersetzung

To distinguish cell division from cell cycle variations in cardiomyocytes, we present protocols using two transgenic mouse lines: Myh6-H2B-mCh transgenic mice, for the unequivocal identification of cardiomyocyte nuclei, and CAG-eGFP-anillin mice, for distinguishing cell division from cell cycle variations.

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