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Developmental Biology

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JoVE Journal Developmental Biology
Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
 

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Article DOI: 10.3791/55204-v • 09:41 min • February 24th, 2017
February 24th, 2017 •
*1, *1, 1, 1, 1
1Institute of Physiology I, University of Bonn
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

To distinguish cell division from cell cycle variations in cardiomyocytes, we present protocols using two transgenic mouse lines: Myh6-H2B-mCh transgenic mice, for the unequivocal identification of cardiomyocyte nuclei, and CAG-eGFP-anillin mice, for distinguishing cell division from cell cycle variations.

Tags

Visualization Cell Cycle Variations Determination Nucleation Postnatal Cardiomyocytes Cardiac Regeneration Technique M Phase Spatiotemporal Resolution Fluorescence Patricia Freitag Technician Transgenic Hearts Fluorescent Microscopy EGFP Analyin H2B-mCherry Expression Isolation Neonatal Heart Dissociation Kit
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