Angiogenesis involves multi-cell, multi-system interactions that need to be investigated in a physiologically relevant environment. The objective of this study is to demonstrate the ability of the rat mesentery culture model to make time-lapse comparisons of intact microvascular networks during angiogenesis.
Azimi, M. S., Motherwell, J. M., Murfee, W. L. An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks. J. Vis. Exp. (120), e55183, doi:10.3791/55183 (2017).