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Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
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Biochemie
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JoVE Journal Biochemie
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

DOI:
10.3791/55154-v

•

08:56 min

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March 25, 2017

•

,
1Department of Chemistry,Boston College

Kapitel

  • 00:05Titel
  • 00:48Preparation of N-TBS-BCl-1,2-azaborine
  • 04:19Protein Crystallization
  • 05:46Complex Preparation
  • 06:58Results: Synthesis, Purification and Complexation of Protein Complexes with T4 Lysozyme Mutants
  • 08:07Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A protocol for the synthesis of 1,2-azaborines and the preparation of their protein complexes with T4 lysozyme mutants is presented.

Tags

12-AzaborinesProtein ComplexesT4 Lysozyme MutantsPalladium On CarbonBoron-11 NMRFlash ChromatographyToluene

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