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Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

DOI:
10.3791/55154-v

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08:56 min

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March 25, 2017

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,
1Department of Chemistry,Boston College

Chapters

  • 00:05Title
  • 00:48Preparation of N-TBS-BCl-1,2-azaborine
  • 04:19Protein Crystallization
  • 05:46Complex Preparation
  • 06:58Results: Synthesis, Purification and Complexation of Protein Complexes with T4 Lysozyme Mutants
  • 08:07Conclusion

Summary

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A protocol for the synthesis of 1,2-azaborines and the preparation of their protein complexes with T4 lysozyme mutants is presented.

Tags

12-AzaborinesProtein ComplexesT4 Lysozyme MutantsPalladium On CarbonBoron-11 NMRFlash ChromatographyToluene

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