Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
와일드 형은 PCR은 낮은 주파수 체세포 돌연변이의 검출을위한 고감도 방법으로 직접 시퀀싱과 결합 블로킹
11,401 Views
•
10:41 min
•
March 29, 2017
DOI:
Summary
Automatically generated
Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.
Read Article
Cite this Article
Albitar, A. Z., Ma, W., Albitar, M. Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations. J. Vis. Exp. (121), e55130, doi:10.3791/55130 (2017).
Copy