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低周波体細胞変異の検出のための高感度な方法として直接配列決定と組み合わせるPCRをブロックする野生型
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Genetik
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JoVE Journal Genetik
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
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低周波体細胞変異の検出のための高感度な方法として直接配列決定と組み合わせるPCRをブロックする野生型

11,400 Views

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10:41 min

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March 29, 2017

DOI:

10.3791/55130-v

DOI:
10.3791/55130-v

•

10:41 min
March 29, 2017

•

4 Views
, ,
1NeoGenomics Laboratories

Summary

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Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.

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Keyword Extraction:Wild-type Blocking PCRSomatic MutationsMyd88 GeneBone Marrow SamplesExon FiveL265 HotspotM13 SequenceBlocking OligonucleotideWild-type TemplateMelting TemperatureExtension TemperatureSecondary StructureSelf-dimerization

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Cite this Article

Albitar, A. Z., Ma, W., Albitar, M. Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations. J. Vis. Exp. (121), e55130, doi:10.3791/55130 (2017).

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