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Entwicklungsbiologie
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制备及文化活动从成年和老年人类骨骼肌成肌前体细胞/成肌细胞主要的
JoVE Journal
Entwicklungsbiologie
This content is Free Access.
JoVE Journal
Entwicklungsbiologie
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
Please note that all translations are automatically generated.
Click here for the English version.
制备及文化活动从成年和老年人类骨骼肌成肌前体细胞/成肌细胞主要的
DOI:
10.3791/55047-v
•
10:10 min
•
February 16, 2017
•
Ana Soriano-Arroquia
,
Peter D. Clegg
,
Andrew P. Molloy
2
,
Katarzyna Goljanek-Whysall
1
Institute of Ageing and Chronic Disease
,
University of Liverpool
,
2
Aintree University Hospital
Kapitel
00:05
Titel
00:47
Culture Preparation
01:40
Tissue Digestion: Mechanical and Enzymatic Dissociation
04:16
Seeding of Cells
05:29
Culture and Passaging of Cells
07:52
Results: Human Primary Myoblasts Characterization and Its Use for Functional Studies of Muscle Homeostasis
In Vitro
09:16
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
本协议描述的隔离和成肌细胞祖细胞培养的成年和老年人的骨骼肌肉健壮,重复性好,简单的方法。这里使用的肌肉包括脚和腿部的肌肉。这种方法使对于功能研究初级成肌细胞的富集群体的分离。
Tags
Myogenic Precursor Cells
Primary Myoblasts
Skeletal Muscle
Adult
Aged
Isolation
In Vitro Model
Muscle Homeostasis
Aging
Laminin
Enzymatic Digestion
DMEM
DPBS
Ethanol
Growth Media
Cell Strainer
Centrifugation
Article
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