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April 26, 2017
DOI:
10.3791/54967-v
该报告介绍了一种生物工程方法来设计和构建特异性调节在哺乳动物细胞中靶基因的剪接新颖人工剪接因子(ASFS)。该方法可以进一步扩展来设计各种人为因素来操纵RNA代谢的其它方面。
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Wei, H., Liu, Y., Wang, Y., Lu, Q., Yang, X., Li, J., Wang, Z. Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells. J. Vis. Exp. (122), e54967, doi:10.3791/54967 (2017).
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