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påvisning af<em> Trypanosoma brucei</em> Variant overfladeglycoprotein Skift af magnetisk cellesortering og flowcytometri
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påvisning af<em> Trypanosoma brucei</em> Variant overfladeglycoprotein Skift af magnetisk cellesortering og flowcytometri
Kapitel
- 00:05Titel
- 01:05Sample Harvesting
- 02:05Magnetic Labeling
- 04:05Magnetic Separation
- 05:45Staining and Identification of Contaminants and Switchers
- 07:32Results: Induced Double-strand DNA Breaks Increase the Frequency of Switchers in a Population
- 09:01Conclusion
African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.
