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Nachweis von<em> Trypanosoma brucei</em> Variante Oberflächen-Glykoprotein Switching von Magnetic Activated Cell Sorting und Durchflusszytometrie
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry
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Nachweis von<em> Trypanosoma brucei</em> Variante Oberflächen-Glykoprotein Switching von Magnetic Activated Cell Sorting und Durchflusszytometrie

8,609 Views

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09:45 min

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October 19, 2016

DOI:

10.3791/54715-v

DOI:
10.3791/54715-v

•

09:45 min
October 19, 2016

•

1 Views
, , ,
1Laboratory of Lymphocyte Biology,Rockefeller University, 2Department of Biology,Harvey Mudd College, 3Masters School

Summary

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African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.

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Schulz, D., Mugnier, M. R., Boothroyd, C. E., Papavasiliou, F. N. Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry. J. Vis. Exp. (116), e54715, doi:10.3791/54715 (2016).

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