Journal
/
Immunologie und Infektion
/
detectie van<em> Trypanosoma brucei</em> Variant oppervlakte glycoproteïne Schakelen van Magnetic Activated Cell Sorting en flowcytometrie
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.

detectie van<em> Trypanosoma brucei</em> Variant oppervlakte glycoproteïne Schakelen van Magnetic Activated Cell Sorting en flowcytometrie

8,610 Views

•

09:45 min

•

October 19, 2016

DOI:

10.3791/54715-v

DOI:
10.3791/54715-v

•

09:45 min
October 19, 2016

•

1 Views
, , ,
1Laboratory of Lymphocyte Biology,Rockefeller University, 2Department of Biology,Harvey Mudd College, 3Masters School

Summary

Automatically generated

African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.

Explore More Videos

Trypanosoma BruceiVariant Surface GlycoproteinVSG SwitchingMagnetic Activated Cell SortingFlow CytometryAntigenic VariationCell IsolationCell EnrichmentLister 427 StrainAnti-VSG AntibodyCell SeparationCell CultureCell CentrifugationCell LabelingMagnetic Beads

Read Article

Cite this Article

Schulz, D., Mugnier, M. R., Boothroyd, C. E., Papavasiliou, F. N. Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry. J. Vis. Exp. (116), e54715, doi:10.3791/54715 (2016).

Download .ris file

Copy

Share Video

.

Copy