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Perturbações de circulação miRNAs na Síndrome do Cólon Irritável detectada usando um multiplexado de alta capacidade: Plataforma Gene Expression
Kapitel
- 00:05Titel
- 00:44Total RNA Purification
- 02:45miRNA Sample Preparation
- 05:18miRNA Hybridization
- 06:47Prep Station and Digital Analyzer
- 08:28Results: High Throughput Gene Expression of miRNAs in Irritable Bowel Syndrome
- 09:57Conclusion
We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.
