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Fare kemik iliğinden plazmasitoid dendritik hücreleri Saflaştırılması floresans ile aktive edilen hücre sınıflandırma
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
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Fare kemik iliğinden plazmasitoid dendritik hücreleri Saflaştırılması floresans ile aktive edilen hücre sınıflandırma

11,779 Views

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08:01 min

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November 04, 2016

DOI:

10.3791/54641-v

DOI:
10.3791/54641-v

•

08:01 min
November 04, 2016

•

13 Views
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1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 2Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Summary

Automatically generated

Biz pDC fonksiyonel çalışmalar için, lupus-eğilimli farelerin kemik iliğinden, yüksek saflıkta plazmasitoid dendritik hücreleri (PDC) izole etmek için sıralama floresans ile aktive edilmiş hücre kullanarak bir protokol sunulmuştur.

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Liao, X., Makris, M., Luo, X. M. Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow. J. Vis. Exp. (117), e54641, doi:10.3791/54641 (2016).

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