Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Kapitel
- 00:05Titel
- 00:49Live Cell Video Microscopy Phagocytosis Assay
- 02:41Analysis of Time Lapse Movies
- 05:29Results: Velocity of Phagosome Migration in HIV-infected and Uninfected Human Macrophages
- 06:20Conclusion
We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.
