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Journal / Biochemistry / Method for Identifying Small Molecule Inhibitors…
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Biochemistry

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JoVE Journal Biochemistry
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
 

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Article DOI: 10.3791/54540-v • 10:20 min • November 11th, 2016
November 11th, 2016 •
*1, *1, 2, 3, 1, 1, 4, 1,5
1Davee Department of Neurology and Clinical Neurosciences, Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery, Northwestern University, 3Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences, Northwestern University, 5Department of Physiology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

Kapitel

Summary
Automatic Translation

The interaction between HCN channels and their auxiliary subunit has been identified as a therapeutic target in Major Depressive Disorder. Here, a fluorescence polarization-based method for identifying small molecule inhibitors of this protein-protein interaction, is presented.

Tags

Small Molecule Inhibitors Protein-protein Interaction HCN1 TRIP8b High Throughput Candidate Molecules New Treatments Depression Therapeutic Benefit Major Depressive Disorder Chronic Pain Visual Demonstration Automated High Throughput Instruments Northwestern University's High Throughput Analysis Lab Purifying Protein Fragment Positive Control FP Buffer Micromolar TRIP8b Nanomolar HCN1-FITC Low Binding 384 Well Black Microtiter Plate
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