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Preparation and Applications of Organotypic Thymic Slice Cultures
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Preparation and Applications of Organotypic Thymic Slice Cultures

Preparation and Applications of Organotypic Thymic Slice Cultures

7,789 Views

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10:10 min

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August 06, 2016

DOI:

10.3791/54355-v

DOI:
10.3791/54355-v

•

10:10 min
August 06, 2016

•

2 Views
, ,
1Centre de Recherche,Hôpital Maisonneuve-Rosemont, 2Department of Microbiology, Infectiology and Immunology,Université de Montréal, 3Department of Medicine,Université de Montréal

Tags

Organotypic Thymic Slice CulturesT Cell DevelopmentPositive And Negative SelectionThymic Cortex And MedullaThymocyte SubsetsThymic MicroenvironmentMouse Thymus DissectionAgarose EmbeddingRPMI-1640 MediaCell Culture Inserts

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Sood, A., Dong, M., Melichar, H. J. Preparation and Applications of Organotypic Thymic Slice Cultures. J. Vis. Exp. (114), e54355, doi:10.3791/54355 (2016).

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