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福尔马林固定石蜡包埋的组织核心的制备RNA和DNA提取
JoVE Journal
Biologie
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JoVE Journal Biologie
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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福尔马林固定石蜡包埋的组织核心的制备RNA和DNA提取

DOI:
10.3791/54299-v

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08:30 min

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August 21, 2016

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, , , , , , , , , ,
1Department of Pathology & Molecular Medicine,Queen’s University, 2Division of Cancer Biology & Genetics, Queen’s Cancer Research Institute,Queen’s University, 3Department of Surgery, Division of Urology,McGill University, 4Transformative Pathology Program,Ontario Institute for Cancer Research (OICR)

Kapitel

  • 00:05Titel
  • 00:57Tissue Coring
  • 02:40Deparaffinization of the FFPE Tissue Cores
  • 03:45Homogenization of the Deparaffinized Cores
  • 05:07Digestion with Proteinase K and Nucleotide Separation
  • 06:12Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis
  • 07:50Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

这个修改​​后的提取方法提高了从组织病理学组织块的兴趣更精确的目标区域的RNA和DNA的产量。

Tags

RNA ExtractionDNA ExtractionProstate CancerParaffin FilmPunch BiopsyXyleneCentrifugation

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