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Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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August 21, 2016
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1Department of Pathology & Molecular Medicine,Queen’s University, 2Division of Cancer Biology & Genetics, Queen’s Cancer Research Institute,Queen’s University, 3Department of Surgery, Division of Urology,McGill University, 4Transformative Pathology Program,Ontario Institute for Cancer Research (OICR)
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This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic tissue blocks.
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Patel, P. G., Selvarajah, S., Boursalie, S., How, N. E., Ejdelman, J., Guerard, K., Bartlett, J. M., Lapointe, J., Park, P. C., Okello, J. B. A., Berman, D. M. Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction. J. Vis. Exp. (114), e54299, doi:10.3791/54299 (2016).
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