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1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center,Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine,Yale University School of Medicine, 3Department of Molecular and Cellular Biology,Baylor College of Medicine
Kapitel
- 00:05Titel
- 01:12Mouse Embryo Yolk Sac (YS) or Aorta-gonad-mesonephros (AGM) Dissection
- 03:29Primary YS/AGM Tissue Digestion
- 04:37Nucleic Acid Dye- and Fluorescently Conjugated Antibody-labeling
- 06:19Results: Representative Flow Cytometric Identification of Hemogenic Endothelial Cells (EC), Hematopoetic Stem and Progenitor Cells (HSPC), and Non-hemogenic EC
- 07:43Conclusion
Hematopoietische stamcellen en progenitorcellen (HSPC) afgeleid van gespecialiseerde (hemogenic) endotheelcellen tijdens de ontwikkeling, maar weinig bekend over het proces waarbij sommige endotheelcellen specificeren bloedvormende worden. We tonen een stroom-cytometrie-gebaseerde methode waarmee gelijktijdige isolatie van hemogenic endotheelcellen en HSPC van muizen embryonale weefsels.
