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Establishment of Cancer Stem Cell Cultures de Human osteossarcoma convencional
JoVE Journal
Krebsforschung
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JoVE Journal Krebsforschung
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Establishment of Cancer Stem Cell Cultures de Human osteossarcoma convencional

18,452 Views

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09:25 min

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October 14, 2016

DOI:

10.3791/53884-v

DOI:
10.3791/53884-v

•

09:25 min
October 14, 2016

•

4 Views
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1Department of Surgery and Translational Medicine (DCMT),University of Florence, 2Neurofarba Department,University of Florence, 3Department of Traumatology and General Orthopedics,Azienda Ospedaliera Universitaria Careggi

Summary

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A presença de células-tronco cancerosas (CSCs) em sarcomas ósseos foi recentemente ligada à sua patogênese. Neste artigo, apresentamos o isolamento de CSCs de culturas de células primárias obtidas a partir de biópsias humanas de osteossarcoma convencional (OS) que utilizam a capacidade dos CSCs a crescer sob condições não aderentes.

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Cite this Article

Palmini, G., Zonefrati, R., Mavilia, C., Aldinucci, A., Luzi, E., Marini, F., Franchi, A., Capanna, R., Tanini, A., Brandi, M. L. Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma. J. Vis. Exp. (116), e53884, doi:10.3791/53884 (2016).

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