Journal
/
/
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
DOI:

08:45 min

March 18, 2016

, , , ,

Kapitel

  • 00:05Titel
  • 00:50Skin Fibroblast Isolation
  • 01:53Fibroblast Culture and Plating for Epigenetic Conversion
  • 04:00Increase Cell Plasticity Using the De-methylating Agent 5-aza-CR
  • 05:31Pancreatic Differentiation Protocol
  • 06:47Results: Morphological and Functional Changes During Epigenetic Conversion
  • 08:02Conclusion

Summary

Automatische Übersetzung

Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.

Verwandte Videos

Read Article